[HTML][HTML] Reversible inactivation of the tumor suppressor PTEN by H2O2
SR Lee, KS Yang, J Kwon, C Lee, W Jeong… - Journal of Biological …, 2002 - ASBMB
The tumor suppressor PTEN regulates cell migration, growth, and survival by removing the
3′-phosphate of phosphoinositides. Exposure of purified PTEN or of cells to H 2 O 2
resulted in inactivation of PTEN in a time-and H 2 O 2 concentration-dependent manner.
Analysis of various cysteine mutants, including mass spectrometry of tryptic peptides,
indicated that the essential Cys 124 residue in the active site of PTEN specifically forms a
disulfide with Cys 71 during oxidation by H 2 O 2. The reduction of H 2 O 2-oxidized PTEN in …
3′-phosphate of phosphoinositides. Exposure of purified PTEN or of cells to H 2 O 2
resulted in inactivation of PTEN in a time-and H 2 O 2 concentration-dependent manner.
Analysis of various cysteine mutants, including mass spectrometry of tryptic peptides,
indicated that the essential Cys 124 residue in the active site of PTEN specifically forms a
disulfide with Cys 71 during oxidation by H 2 O 2. The reduction of H 2 O 2-oxidized PTEN in …