PURPOSE

A 28-color panel was developed to screen for a range of lymphocyte subsets in human peripheral blood mononuclear cells (PBMCs), particularly in patients with primary immunodeficiency (PID). Using this panel, we are able to avoid running the sample over multiple screening panels while still deeply phenotyping a diverse range of lymphocyte subsets including innate like lymphocytes (γδ, mucosalassociated invariant T [MAIT], natural killer [NK], and NKT cells), as well as multiple subsets of naïve and memory CD4+ and CD8+ T cells, and B cells. Specifically, naïve, central memory (cmem) and effector memory (emem) CD4+ T cells, naïve, cmem, emem and CD45RA+ revertant memory (TEMRA) CD8+ T cells, regulatory (Tregs), T follicular helper (Tfh) and T helper (Th) 1, and Th17 CD4+ T cells, and transitional, naïve, memory, and CD21lo B cells.