Effect of strain on bone nodule formation by rat osteogenic cells in vitro
LA Visconti, EHK Yen, RB Johnson - Archives of Oral Biology, 2004 - Elsevier
The purpose of this study was to assess in vitro bone nodule formation by cells exposed to a
range of microstrain, at a sub-optimal oscillation frequency for bone formation. Fetal rat
calvarial cells experienced a Flexercell™ regimen within either FLEX I™(deformable) or
FLEX II™(non-deformable) substrates. Cells in FLEX I™ plates were exposed to growth
medium only; those in FLEX II™ plates were exposed to either growth medium only, or
growth medium+ 10− 7M IGF-1. Cell numbers were assessed from 1 to 6 days. Other cells …
range of microstrain, at a sub-optimal oscillation frequency for bone formation. Fetal rat
calvarial cells experienced a Flexercell™ regimen within either FLEX I™(deformable) or
FLEX II™(non-deformable) substrates. Cells in FLEX I™ plates were exposed to growth
medium only; those in FLEX II™ plates were exposed to either growth medium only, or
growth medium+ 10− 7M IGF-1. Cell numbers were assessed from 1 to 6 days. Other cells …