[HTML][HTML] Imaging regulatory T cell dynamics and CTLA4-mediated suppression of T cell priming

MP Matheu, S Othy, ML Greenberg, TX Dong… - Nature …, 2015 - nature.com
MP Matheu, S Othy, ML Greenberg, TX Dong, M Schuijs, K Deswarte, H Hammad…
Nature communications, 2015nature.com
Foxp3+ regulatory T cells (Tregs) maintain immune homoeostasis through mechanisms that
remain incompletely defined. Here by two-photon (2P) imaging, we examine the cellular
dynamics of endogenous Tregs. Tregs are identified as two non-overlapping populations in
the T-zone and follicular regions of the lymph node (LN). In the T-zone, Tregs migrate more
rapidly than conventional T cells (Tconv), extend longer processes and interact with resident
dendritic cells (DC) and Tconv. Tregs intercept immigrant DCs and interact with antigen …
Abstract
Foxp3+ regulatory T cells (Tregs) maintain immune homoeostasis through mechanisms that remain incompletely defined. Here by two-photon (2P) imaging, we examine the cellular dynamics of endogenous Tregs. Tregs are identified as two non-overlapping populations in the T-zone and follicular regions of the lymph node (LN). In the T-zone, Tregs migrate more rapidly than conventional T cells (Tconv), extend longer processes and interact with resident dendritic cells (DC) and Tconv. Tregs intercept immigrant DCs and interact with antigen-induced DC:Tconv clusters, while continuing to form contacts with activated Tconv. During antigen-specific responses, blocking CTLA4-B7 interactions reduces Treg–Tconv interaction times, increases the volume of DC:Tconv clusters and enhances subsequent Tconv proliferation in vivo. Our results demonstrate a role for altered cellular choreography of Tregs through CTLA4-based interactions to limit T-cell priming.
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