[HTML][HTML] CD41-YFP mice allow in vivo labeling of megakaryocytic cells and reveal a subset of platelets hyperreactive to thrombin stimulation
J Zhang, F Varas, M Stadtfeld, S Heck, N Faust… - Experimental …, 2007 - Elsevier
OBJECTIVE: Development of a mouse line permitting live imaging of cells expressing
CD41/GpIIb as a means to study megakaryopoiesis. MATERIALS AND METHODS: The
gene encoding yellow fluorescent protein (eyfp) was inserted by homologous recombination
into embryonic stem cells at the start site of the gpIIb locus. A knockin mouse line,
designated CD41-yellow fluorescent protein (YFP), was developed and was characterized
by fluorescence microscopy and flow cytometry. Activity of YFP+ platelets was determined by …
CD41/GpIIb as a means to study megakaryopoiesis. MATERIALS AND METHODS: The
gene encoding yellow fluorescent protein (eyfp) was inserted by homologous recombination
into embryonic stem cells at the start site of the gpIIb locus. A knockin mouse line,
designated CD41-yellow fluorescent protein (YFP), was developed and was characterized
by fluorescence microscopy and flow cytometry. Activity of YFP+ platelets was determined by …