Translational profiles of medullary myofibroblasts during kidney fibrosis

I Grgic, AM Krautzberger, A Hofmeister… - Journal of the …, 2014 - journals.lww.com
Myofibroblasts secrete matrix during chronic injury, and their ablation ameliorates fibrosis.
Development of new biomarkers and therapies for CKD will be aided by a detailed analysis
of myofibroblast gene expression during the early stages of fibrosis. However, dissociating
myofibroblasts from fibrotic kidney is challenging. We therefore adapted translational
ribosome affinity purification (TRAP) to isolate and profile mRNA from myofibroblasts and
their precursors during kidney fibrosis. We generated and characterized a transgenic mouse …