Multiphoton microscopy applied for real-time intravital imaging of bacterial infections in vivo

FX Choong, RM Sandoval, BA Molitoris… - Methods in …, 2012 - Elsevier
To understand the underlying mechanisms of bacterial infections, researchers have for long
addressed the molecular interactions occurring when the bacterium interacts with host target
cells. In these studies, primarily based on in vitro systems, molecular details have been
revealed along with increased knowledge regarding the general infection process. With the
recent advancements in in vivo imaging techniques, we are now in a position to bridge a
transition from classical minimalistic in vitro approaches to allow infections to be studied in …