Purification of an N-ethylmaleimide-sensitive protein catalyzing vesicular transport.
MR Block, BS Glick, CA Wilcox… - Proceedings of the …, 1988 - National Acad Sciences
MR Block, BS Glick, CA Wilcox, FT Wieland, JE Rothman
Proceedings of the national academy of sciences, 1988•National Acad SciencesN-Ethylmaleimide (NEM) inhibits protein transport between successive compartments of the
Golgi stack in a cell-free system. After inactivation of the Golgi membranes by NEM, transport
can be rescued by adding back an appropriately prepared cytosol fraction. This
complementation assay has allowed us to purify the NEM-sensitive factor, which we term
NSF. The NEM-sensitive factor is a tetramer of 76-kDa subunits, and appears to act
catalytically, one tetramer leading to the metabolism of numerous transport vesicles.
Golgi stack in a cell-free system. After inactivation of the Golgi membranes by NEM, transport
can be rescued by adding back an appropriately prepared cytosol fraction. This
complementation assay has allowed us to purify the NEM-sensitive factor, which we term
NSF. The NEM-sensitive factor is a tetramer of 76-kDa subunits, and appears to act
catalytically, one tetramer leading to the metabolism of numerous transport vesicles.
N-Ethylmaleimide (NEM) inhibits protein transport between successive compartments of the Golgi stack in a cell-free system. After inactivation of the Golgi membranes by NEM, transport can be rescued by adding back an appropriately prepared cytosol fraction. This complementation assay has allowed us to purify the NEM-sensitive factor, which we term NSF. The NEM-sensitive factor is a tetramer of 76-kDa subunits, and appears to act catalytically, one tetramer leading to the metabolism of numerous transport vesicles.
National Acad Sciences