Vasopressin regulates water reabsorption in the collecting duct, but extracellular nucleotides
modulate this regulation through incompletely understood mechanisms. We investigated
these mechanisms using immortalized mouse collecting duct (mpkCCD) cells. Basolateral
exposure to dDAVP induced AQP2 localization to the apical membrane, but co-treatment
with ATP internalized AQP2. Because plasma membrane-bound P2 receptors (P2R)
mediate the effects of extracellular nucleotides, we examined the abundance and …