Luminal P2Y2 receptor-mediated inhibition of Na+ absorption in isolated perfused mouse CCD
H Lehrmann, SJ Kim, C Jacobi… - Journal of the American …, 2002 - journals.lww.com
Extracellular nucleotides regulate renal transport. A luminal P2Y 2 receptor in mouse cortical
collecting duct (CCD) principal cells has been demonstrated elsewhere. Herein the effects of
adenosine triphosphate (ATP) and uridine triphosphate (UTP) on electrogenic Na+
absorption in perfused CCD of mice kept on a low-NaCl diet were investigated.
Simultaneously, transepithelial voltage (V te), transepithelial resistance (R te), and fura-2
[Ca 2+] i fluorescence were measured. Baseline parameters were V te,− 16.5±1.2 mV; R te …
collecting duct (CCD) principal cells has been demonstrated elsewhere. Herein the effects of
adenosine triphosphate (ATP) and uridine triphosphate (UTP) on electrogenic Na+
absorption in perfused CCD of mice kept on a low-NaCl diet were investigated.
Simultaneously, transepithelial voltage (V te), transepithelial resistance (R te), and fura-2
[Ca 2+] i fluorescence were measured. Baseline parameters were V te,− 16.5±1.2 mV; R te …