PURPOSE: To define which intracellular pools of sphingomyelin and ceramide are involved
in the triggering of apoptosis of Jurkat leukemia cells in response to γ-ray exposure.
METHODS AND MATERIALS: We examined the kinetics of ceramide generation at the
whole-cell level and in different subcellular compartments (plasma membrane rafts,
mitochondria, and endoplasmic reticulum) after irradiation with photons. Ceramide was
measured by high-performance liquid chromatography or after pulse labeling experiments …