Simultaneous detection of cucumber mosaic virus, tomato mosaic virus and potato virus Y by flow cytometry
D Iannelli, L D'Apice, C Cottone, M Viscardi… - Journal of virological …, 1997 - Elsevier
The simultaneous detection is described of cucumber mosaic virus (CMV), potato virus Y
(PVY) and tomato mosaic virus (ToMV) by flow cytometry. Extracts from leaves of healthy
and CMV or PVY infected plants were incubated with latex particles, each with a diameter of
3 μm. Extracts from ToMV infected or uninfected plants, however, were incubated with
particles, each with a diameter of 6 μm. Beads were washed and incubated in succession
with primary and secondary antibodies, the latter labeled with phycoerythrin (PE) or …
(PVY) and tomato mosaic virus (ToMV) by flow cytometry. Extracts from leaves of healthy
and CMV or PVY infected plants were incubated with latex particles, each with a diameter of
3 μm. Extracts from ToMV infected or uninfected plants, however, were incubated with
particles, each with a diameter of 6 μm. Beads were washed and incubated in succession
with primary and secondary antibodies, the latter labeled with phycoerythrin (PE) or …
