[HTML][HTML] Controlling epidermal growth factor (EGF)-stimulated Ras activation in intact cells by a cell-permeable peptide mimicking phosphorylated EGF receptor
M Rojas, SY Yao, YZ Lin - Journal of Biological Chemistry, 1996 - ASBMB
Epidermal growth factor (EGF)-stimulated Ras activation involves specific interactions
between the EGF receptor (EGFR), the adaptor proteins Grb2 and Shc, and the nucleotide
exchange factor Sos-1. Study and control of these protein-protein interactions in vivo can be
greatly promoted by introducing intracellular reagents that mimic EGFR functions. Here, we
showed that a synthetic phosphopeptide encompassing the autophosphorylation site 1068
of EGFR formed a complex with endogenous Grb2 after this peptide was delivered into intact …
between the EGF receptor (EGFR), the adaptor proteins Grb2 and Shc, and the nucleotide
exchange factor Sos-1. Study and control of these protein-protein interactions in vivo can be
greatly promoted by introducing intracellular reagents that mimic EGFR functions. Here, we
showed that a synthetic phosphopeptide encompassing the autophosphorylation site 1068
of EGFR formed a complex with endogenous Grb2 after this peptide was delivered into intact …