Phenotypic characterization and functional analysis of human tumor immune infiltration after mechanical and enzymatic disaggregation
C Grange, J Létourneau, MA Forget… - Journal of …, 2011 - Elsevier
Multi-parametric flow cytometry analysis is a reliable method for phenotypic and functional
characterization of tumor infiltrating immune cells (TIIC). The isolation of infiltrating
leukocytes from solid tumors can be achieved through various methods which can be both
enzymatic and mechanical; however, these methods may alter cell biology. The aim of this
study was to compare the effects of three tissue disaggregation techniques on TIIC biology
in breast, kidney and lung tumor specimens. We therefore compared two enzymatic …
characterization of tumor infiltrating immune cells (TIIC). The isolation of infiltrating
leukocytes from solid tumors can be achieved through various methods which can be both
enzymatic and mechanical; however, these methods may alter cell biology. The aim of this
study was to compare the effects of three tissue disaggregation techniques on TIIC biology
in breast, kidney and lung tumor specimens. We therefore compared two enzymatic …
