Quantitative real-time polymerase chain reaction measurement of regulators of G-protein signaling mRNA levels in mouse tissues
DM Kurrasch, J Huang, TM Wilkie, JJ Repa - Methods in enzymology, 2004 - Elsevier
Regulators of G-protein signaling (RGS) play a critical role in G-protein-coupled receptor
signaling in mammalian cells. RGS proteins are GTPase-accelerating proteins (GAPs) for α
subunits of heterotrimeric G proteins of the G i and G q class. RGS GAPs can modulate the
frequency and duration of G-protein signaling and may constitute a new family of therapeutic
targets. Identifying the tissue distribution and cellular localization of RGS proteins has been
hindered by the lack of effective antibodies for immunodetection. The measurement of …
signaling in mammalian cells. RGS proteins are GTPase-accelerating proteins (GAPs) for α
subunits of heterotrimeric G proteins of the G i and G q class. RGS GAPs can modulate the
frequency and duration of G-protein signaling and may constitute a new family of therapeutic
targets. Identifying the tissue distribution and cellular localization of RGS proteins has been
hindered by the lack of effective antibodies for immunodetection. The measurement of …
