Dkk-1 secreted by mesenchymal stem cells inhibits growth of breast cancer cells via depression of Wnt signalling
L Qiao, Z Xu, T Zhao, L Ye, X Zhang - Cancer letters, 2008 - Elsevier
L Qiao, Z Xu, T Zhao, L Ye, X Zhang
Cancer letters, 2008•ElsevierWe determined the molecular mechanism of inhibitory effect of human mesenchymal stem
cells (hMSCs) on the growth of human MCF-7 breast cancer cells. Our finding showed that β-
catenin was down-regulated in MCF-7 cells by conditioned media from Z3 hMSCs, and the
expression level of dickkopf-1 (Dkk-1) was higher in Z3 cells than that in MCF-7 cells.
Neutralization of Dkk-1 and small interference RNA targeting Dkk-1 mRNA in Z3 cells
attenuated the inhibitory effect of Z3 cells on MCF-7 cells. Overexpression of Dkk-1 in Z3 …
cells (hMSCs) on the growth of human MCF-7 breast cancer cells. Our finding showed that β-
catenin was down-regulated in MCF-7 cells by conditioned media from Z3 hMSCs, and the
expression level of dickkopf-1 (Dkk-1) was higher in Z3 cells than that in MCF-7 cells.
Neutralization of Dkk-1 and small interference RNA targeting Dkk-1 mRNA in Z3 cells
attenuated the inhibitory effect of Z3 cells on MCF-7 cells. Overexpression of Dkk-1 in Z3 …
We determined the molecular mechanism of inhibitory effect of human mesenchymal stem cells (hMSCs) on the growth of human MCF-7 breast cancer cells. Our finding showed that β-catenin was down-regulated in MCF-7 cells by conditioned media from Z3 hMSCs, and the expression level of dickkopf-1 (Dkk-1) was higher in Z3 cells than that in MCF-7 cells. Neutralization of Dkk-1 and small interference RNA targeting Dkk-1 mRNA in Z3 cells attenuated the inhibitory effect of Z3 cells on MCF-7 cells. Overexpression of Dkk-1 in Z3 cells enhanced the inhibition. Therefore, Dkk-1 secreted by Z3 cells involves the inhibition via the Wnt pathway.
Elsevier
