Improved assay for plasma dihydroxyphenylacetic acid and other catechols using high-performance liquid chromatography with electrochemical detection

C Holmes, G Eisenhofer, DS Goldstein - Journal of Chromatography B …, 1994 - Elsevier
Several modifications of an HPLC—electrochemical assay method for plasma levels of
norepinephrine (NE), epinephrine (EPI), dopamine (DA), dihydroxyphenylglycol (DHPG),
dihydroxyphenylalanine (DOPA) and dihydroxyphenylacetic acid (DOPAC) that improve the
accuracy and reliability of DHPG, DOPA, and DOPAC measurements are described. In batch
alumina extractions, increasing the amount of alumina decreased analytical recoveries of
DHPG, DOPA, and especially DOPAC, and increasing the strength of the eluting acid …