Measurement of immunoreactive interleukin-1β from human mononuclear cells: optimization of recovery, intrasubject consistency, and comparison with interleukin-1α …
S Endres, R Ghorbani, G Lonnemann… - Clinical immunology and …, 1988 - Elsevier
Numerous studies have reported altered levels of in vitro production of the cytokines
interleukin-1 (IL-1) and tumor necrosis factor (TNF) from blood leukocytes in various human
disease states. Most of these studies have used bioassays which are vulnerable to inhibitors
produced by these cells. Furthermore in vitro cytokine production is often assessed on a
single occasion. The present study was designed to standardize stimulation conditions for in
vitroIL-1β production and to employ a competitive radioimmunoassay (RIA) to demonstrate …
interleukin-1 (IL-1) and tumor necrosis factor (TNF) from blood leukocytes in various human
disease states. Most of these studies have used bioassays which are vulnerable to inhibitors
produced by these cells. Furthermore in vitro cytokine production is often assessed on a
single occasion. The present study was designed to standardize stimulation conditions for in
vitroIL-1β production and to employ a competitive radioimmunoassay (RIA) to demonstrate …