ASP enhances in situ lipoprotein lipase activity by increasing fatty acid trapping in adipocytes
M Faraj, AD Sniderman, K Cianflone - Journal of Lipid Research, 2004 - ASBMB
Acylation-stimulating protein (ASP) increases triglyceride (TG) storage (fatty acid trapping) in
adipose tissue and plays an important role in postprandial TG clearance. We examined the
capacity of ASP and insulin to stimulate the activity of lipoprotein lipase (LPL) and the
trapping of LPL-derived nonesterified fatty acid (NEFA) in 3T3-L1 adipocytes. Although
insulin increased total LPL activity (secreted and cell-associated; P< 0.001) in 3T3-L1
adipocytes, ASP moderately stimulated secreted LPL activity (P= 0.04; 5% of total LPL …
adipose tissue and plays an important role in postprandial TG clearance. We examined the
capacity of ASP and insulin to stimulate the activity of lipoprotein lipase (LPL) and the
trapping of LPL-derived nonesterified fatty acid (NEFA) in 3T3-L1 adipocytes. Although
insulin increased total LPL activity (secreted and cell-associated; P< 0.001) in 3T3-L1
adipocytes, ASP moderately stimulated secreted LPL activity (P= 0.04; 5% of total LPL …
