Rapid isolation of Tamm-Horsfall glycoprotein (uromodulin) from human urine
F Serafini-Cessi, G Bellabarba, N Malagolini… - Journal of …, 1989 - Elsevier
An isolation method for Tamm-Horsfall protein is described which is based on the
observation that a diatomaceous earth filter is able to retain most of the glycoprotein present
in urine and that the glycoprotein is easily desorbed from the filter by deionized water. This
behaviour depends on the tendency of Tamm-Horsfall glycoprotein at normal urinary
concentrations to form a gel in a solution containing mono-and divalent ions. By means of
two-step filtration, the glycoprotein was purified to homogeneity. The yield was of about 20 …
observation that a diatomaceous earth filter is able to retain most of the glycoprotein present
in urine and that the glycoprotein is easily desorbed from the filter by deionized water. This
behaviour depends on the tendency of Tamm-Horsfall glycoprotein at normal urinary
concentrations to form a gel in a solution containing mono-and divalent ions. By means of
two-step filtration, the glycoprotein was purified to homogeneity. The yield was of about 20 …
