[HTML][HTML] Elevated function of blood clotting factor VIIa mutants that have enhanced affinity for membranes: behavior in a diffusion-limited reaction
GL Nelsestuen, M Stone, MB Martinez… - Journal of Biological …, 2001 - ASBMB
Blood clotting factor VIIa is involved in the first step of the blood coagulation cascade, as a
membrane-associated enzyme in complex with tissue factor (TF). Factor VIIa is also an
important therapeutic agent for hemophilia where its function may include TF-independent
as well as TF-dependent mechanisms. This study compared the activity of wild type factor
VIIa (WT-VIIa) with that of a mutant with elevated affinity for membrane (P10Q/Q32E, QE-
VIIa). Phospholipid and cell-based assays showed the mutant to have up to 40-fold higher …
membrane-associated enzyme in complex with tissue factor (TF). Factor VIIa is also an
important therapeutic agent for hemophilia where its function may include TF-independent
as well as TF-dependent mechanisms. This study compared the activity of wild type factor
VIIa (WT-VIIa) with that of a mutant with elevated affinity for membrane (P10Q/Q32E, QE-
VIIa). Phospholipid and cell-based assays showed the mutant to have up to 40-fold higher …
