Idiotypic Vaccine for Treatment of Human B-Cell Lymphoma: Construction of IgG Variable Regions from Single Malignant B Cells
P Terness, M Welschof, G Moldenhauer, M Jung… - Human immunology, 1997 - Elsevier
Immunoglobulin idiotypes (Id) of malignant B cells represent highly specific markers which
can be used for vaccination. PCR-amplification of immunoglobulin genes enables the rapid
production of large amounts of Id vaccines. However, the separate amplification and
subsequent recombination of heavy and light chains can lead to a loss of the relevant Id. To
preserve the original chain pairs, we used single malignant B cells derived from an
immunocytoma patient. Cytoplasm was extracted and the mRNA transcribed into cDNA. The …
can be used for vaccination. PCR-amplification of immunoglobulin genes enables the rapid
production of large amounts of Id vaccines. However, the separate amplification and
subsequent recombination of heavy and light chains can lead to a loss of the relevant Id. To
preserve the original chain pairs, we used single malignant B cells derived from an
immunocytoma patient. Cytoplasm was extracted and the mRNA transcribed into cDNA. The …