[HTML][HTML] 13C/31P NMR assessment of mitochondrial energy coupling in skeletal muscle of awake fed and fasted rats: relationship with uncoupling protein 3 …

BM Jucker, J Ren, S Dufour, X Cao, SF Previs… - Journal of Biological …, 2000 - ASBMB
To examine the relationship between mitochondrial energy coupling in skeletal muscle and
change in uncoupling protein 3 (UCP3) expression during the transition from the fed to
fasted state, we used a novel noninvasive 31 P/13 C NMR spectroscopic approach to
measure the degree of mitochondrial energy coupling in the hind limb muscles of awake rats
before and after a 48-h fast. Compared with fed levels, UCP3 mRNA and protein levels in
the gastrocnemius increased 1.7-(p< 0.01) and 2.9-fold (p< 0.001), respectively, following a …