Nitric oxide mediates interferon-gamma-induced hyperpermeability in cultured human intestinal epithelial monolayers
N Unno, MJ Menconi, M Smith, MP Fink - Critical care medicine, 1995 - journals.lww.com
Objective Incubation with interferon-gamma has been shown to increase the permeability of
cultured monolayers of intestinal epithelial cells. We sought to determine whether this
phenomenon is mediated, at least in part, by increased production of nitric oxide. Design
Prospective, controlled, laboratory study. Human intestinal epithelial (Caco-2BBe) cells were
grown as monolayers on permeable supports mounted in bicameral chambers. Permeability
was assessed by adding fluorescein sulfonic acid (molecular weight= 478 daltons) to the …
cultured monolayers of intestinal epithelial cells. We sought to determine whether this
phenomenon is mediated, at least in part, by increased production of nitric oxide. Design
Prospective, controlled, laboratory study. Human intestinal epithelial (Caco-2BBe) cells were
grown as monolayers on permeable supports mounted in bicameral chambers. Permeability
was assessed by adding fluorescein sulfonic acid (molecular weight= 478 daltons) to the …
