Electron tomography of neuronal mitochondria: three-dimensional structure and organization of cristae and membrane contacts
G Perkins, C Renken, ME Martone, SJ Young… - Journal of structural …, 1997 - Elsevier
The structure of neuronal mitochondria from chick and rat was examined using electron
microscope tomography of chemically fixed tissue embedded in plastic and sliced in≈ 500-
nm-thick sections. Three-dimensional reconstructions of representative mitochondria were
made from single-axis tilt series acquired with an intermediate voltage electron microscope
(400 kV). The tilt increment was either 1° or 2° ranging from− 60° to+ 60°. The mitochondrial
ultrastructure was similar across species and neuronal regions. The outer and inner …
microscope tomography of chemically fixed tissue embedded in plastic and sliced in≈ 500-
nm-thick sections. Three-dimensional reconstructions of representative mitochondria were
made from single-axis tilt series acquired with an intermediate voltage electron microscope
(400 kV). The tilt increment was either 1° or 2° ranging from− 60° to+ 60°. The mitochondrial
ultrastructure was similar across species and neuronal regions. The outer and inner …
